The effect of a candidate feed additive derived from the essential oils of Pinus merkusii (jungh. & de vriese) and Melaleuca leucadendra (l.) on the kinetics of gas production and methane emitted during in-vitro ruminal fermentation

The study was designed to determine the effect of a candidate natural feed additive on kinetics gas production as representation degradability and methane produced during rumen fermentation. Three blends essential oil (BEO) candidates for additives were formulated using pine eucalyptus oils in following ratios: 75:25, 50:50, 25:75 BEO1, BEO2, BEO3, respectively. Every BEO added batch fermentation system at dosages 0, 100, 200 l/l medium. Furthermore, an vitro technique used simulate According kinetics, all did not affect total potential produced, well from soluble insoluble substrate. rates similar among treatments. addition volume Meanwhile, BEO1 dose 3 100 significantly reduced (P0.05). In conclusion, l/l, respectively, had reduce without negative nutrient digestibility.